By

Dr. Rita Marouga

Global Product Manager

Abstract:

Ettan™ DIGE systems use 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE), the benchmark for protein abundance analysis. The world’s leading pharmaceutical and academic centers of excellence are increasingly switching to Ettan DIGE because of the unrivaled accuracy they provide.

Standardize your 2-D results:
Ettan DIGE uses multiplexing, the simultaneous coseparation of multiple, fluorescently labeled samples, including an internal standard on each gel. Each protein spot has its own internal standard, which ensures that the differences you see in protein abundance are real. This is the only effective way to minimize gel-to-gel variation and significantly increase accuracy and reproducibility.

Efficiency saves time and costs:
The level of reproducibility and statistical confidence with Ettan DIGE ensures that you get results you can rely on every time. You can also be confident that you’re not missing important differences in protein abundance. Because Ettan DIGE requires far fewer gels than other methods for measuring protein abundance differences, it also provides significant time and cost savings.