Speaker:

Dr. Ray Meng
International Field Application Specialist
Gene Expression Division, Bio-Rad USA

Part 1:

The Most Out of Your Real-time Experiments

While real-time RT-PCR has a tremendous potential for analytical and quantitative applications, a comprehensive understanding of its underlying principles is important. In this talk, experts in the field will present a comprehensive guide to the most up-to-date technologies and applications as well as providing an overview of the theory of this increasingly important technique. Topics include assay design, primer design, temperature ramping rate, reaction efficiency, fast real time PCR, relative quantification: important parameters for successful experiments.

Part 2:

High Resolution Melt (HRM) – the Theory, Applications + Demo

When it comes to genotyping and mutation scanning, high-resolution DNA melting is emerging as the technique of choice because it is inexpensive, simple, accurate and rapid. Development of this method of DNA analysis has been underway since its introduction in 2002 by a team of researchers from Pathology Department led by Dr. Carl Wittwer and Dr. Karl Voelkerding at the University of Utah coupled with collaborative efforts from Idaho Technology.

In this seminar, the fundamentals, applications, and the workflow (including assay design and data analysis, etc.) using Bio-Rad Real-time qPCR instrument, reagent, and software will be introduced.