Efficient and Specific Quantification of Mammalian MicroRNAs Using a Novel Real-Time PCR Approach
Dr. Constanze Kindler
Senior Global Product Manager
on Friday, 7 September 2007 at 3:30p.m.
Genome Research Centre
21 Sassoon Road, Pokfulam, Hong Kong
This seminar will introduce a method for efficient and accurate quantification of micro RNAs and other non-coding RNAs by real-time PCR using SYBR Green based detection system. This system is highly specific and sensitive, and requires very small amounts of input RNA. It enables detection of miRNAs as well as mRNAs using the same cDNA preparation allowing simultaneous quantification of miRNA and target mRNA.
Single cDNA prep is sufficient for quantification of several miRNAs of interest, avoiding the need to prepare multiple cDNA preps to quantify each miRNA. Use of a single prep eliminates potential variation that could arise during multiple cDNA synthesis reactions. We will demonstrate the application of this technology to miRNA expression profiling in a model human cell-culture system.
This technology offers researchers a sensitive, specific, and easy-to-perform approach for accurate expression profiling of miRNAs using a small amount of total RNA containing miRNAs. A single cDNA prep from a precious RNA sample is sufficient for profiling of all the known miRNAs in a given model system. This is a substantial advance in the state of the art and would be of broad interest to all scientists studying miRNAs and their targets.