Congratulations to our latest 454 run with superb quality!
Contact us for your exciting 454 projects soon!

	Roche's specifications	Latest in-house run data (4-May-2012)
Number of reads	1 million	1.27 million
Modal read length	450 bp	500 bp
Throughput	450 Mb	527 Mb

Interested in our other Next Generation Sequencing-related instruments? 
Check them out at NGS latest updates.

We are pleased to announce the launch of CLC Genomics Workbench software to assist users in analysing next generation sequencing (NGS) data. The software is currently installed on a dedicated Mac Pro with 2 x Quad core running at 2.8Ghz, 8 Gb of RAM and 30” inch of Apple Cinema Display. 

Software tutorial session is available to help you get started.

For more information, visit our Bioinformatics tools website.

The CGS continues to host 3 different NGS-related instruments, of which 2 are for sequencing and 1 for target enrichment.

LOW COST trial runs are ready to start in March 2012.

We accept BOTH customized collaborative or standard core service work!

And YES, we would gladly provide bioinformatics support as well! 

What are the 3 instruments?

Genome Analyzer IIx	GS FLX Titanium XLR70	RDT 1000
Solexa	454	RainDance
by Illumina	by Roche	by RainDance
Sequencing By Synthesis	Pyrosequencing	Microdroplet-based PCR amplification
Read length up to 150 bp	Mode read length ~ 450 bp	Amplicon size 500-1500bp Up to 20,000 PCR in a single tube
Data output depends on run type 1 x 35 bp = 10-12 G bases / run 2 x 150 bp = 85-95 G bases / run (and variable in-between)	Typical data output 450 M bases / run	Target amplified region 10Kb - 10Mb
7 samples / run (without indexing)	2/4/8/16 samples / run (without indexing)	1 sample / run
Run time 2-14 days	Run time 10 hours	Run time <1 hr (for microdroplet mixing)

Which sequencer is suitable for my work?

Application	Solexa	454	What Might Be Your Questions?
Exome sequencing	+++	+	Are there novel mutations in the exonic sequences?
RNA-seq	+++	+	What genes are differentially expressed in my samples? Are there different splicing patterns between my samples?
ChIP-seq	+++	+	What sequences do my protein binds to?
de novo whole genome sequencing	+++	+++	How does the genome of this organisim look like?
Resequencing	+++	++	What are the sequence differences between my isolate of bacteria/virus/plasmid compare to the common strains?
Metagenomics	+	+++	What micro-organisms are present in this environmental sample? What are the different virus strains in this culture?
MicroRNA profiling	+++	-	What microRNAs are expressed, or differentially expressed in my samples?
Methylation analysis	+++	-	Which sites are methylated in the genome? Is the methylation pattern different between my samples?

Contact us NOW to reserve your low cost trial slots!

Prof Pak Sham
Director
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2831-5425

Dr Agnes Chan
Manager
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2831-5427