Bio-Plex Suspension Array System is based on the Luminex’s xMAP technology.  It permits the simultaneous analysis of up to 100 different biomolecules (proteins, peptides or nucleic acids) in a single well.

Currently, the CGS uses the Bio-Plex 200 system with Bio-Plex Manager 5.0 Software to provide core services.

The technology can be applied to protein expression profiling such as quantitative measurement of cytokines.

Advantage of Luminex’s xMAP technology in comparison to ELISA

	ELISA	xMAP
Number of kit / plate / run	25	1
Serum volume required	625uL 1	25uL
Handling time	100 hours 2	8 hours
Cost of the reagents	HK$ 50,000 - HK$ 100,000 3	HK$ 33,700

Notes:
¹ 25uL for each run
² The average handling time for one ELISA kit is 4 hours
³ The cost for one ELISA kit is HK$ 2,000 - HK$ 4,000

For detail information about xMAP technology, please visit the Luminex website or review CGS past seminars

Different suppliers provide different packages for cytokines screening.

Below are the major xMAP technology compatible reagent kit suppliers, whose kits have been tested at the CGS:

• Affymetrix Panomics
• Bio-Rad
• Invitrogen
• Millipore